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Technical Notes and Downloads

Bioengineered Human Skeletal Muscle as a Platform to Model Injury, Regeneration and Drug Response.

Technical Note #1: Barium Chloride (BaCl2): Isomorphic Mimicry Mechanism

axoCells® iPSC-Derived Motor Neurons as a Platform for Human
Neuromuscular Junction Modelling

Publications: Platform Development

Fleming, J.W., Capel, A.J., Rimington, R.P., Wheeler, P., Leonard, A.N., Bishop, N.C., Davies, O.G., Lewis, M.P. Bioengineered human skeletal muscle capable of functional regeneration. BMC Biol 18, 145 (2020). https://doi.org/10.1186/s12915-020-00884-3
Rimington, R.P., Fleming, J.W., Capel, A.J., Wheeler, P., Lewis, M.P. Bioengineered model of the human motor unit with physiologically functional neuromuscular junctions. Sci Rep 11, 11695 (2021). https://doi.org/10.1038/s41598-021-91203-5
Capel Andrew J. , Rimington Rowan P. , Fleming Jacob W. , Player Darren J. , Baker Luke A. , Turner Mark C. , Jones Julia M. , Martin Neil R. W. , Ferguson Richard A. , Mudera Vivek C. , Lewis Mark P. Scalable 3D Printed Molds for Human Tissue Engineered Skeletal Muscle. Frontiers in Bioengineering and Biotechnology. 2019; 7. https://doi.org/10.3389/fbioe.2019.00020

Publications: Platform Applications

Aguilar-Agon KW, Capel AJ, Martin NR, Player DJ, and Lewis MP. Mechanical loading stimulates hypertrophy in tissue-engineered skeletal muscle: Molecular and phenotypic responses. J Cell Physiol. 2019; 234: 23547–23558. https://doi.org/10.1002/jcp.28923
Jones Julia M. , Player Darren J. , Martin Neil R. W. , Capel Andrew J. , Lewis Mark P., Mudera Vivek. An Assessment of Myotube Morphology, Matrix Deformation, and Myogenic mRNA Expression in Custom-Built and Commercially Available Engineered Muscle Chamber Configurations. Frontiers in Physiology. 2018.9. https://doi.org/10.3389/fphys.2018.00483
Fleming JW, Capel AJ, Rimington RP, Player DJ, Stolzing A, Lewis MP. Functional regeneration of tissue engineered skeletal muscle in vitro is dependent on the inclusion of basement membrane proteins. Cytoskeleton. 2019; 76: 371–382. https://doi.org/10.1002/cm.21553
Aguilar-Agon, K.W., Capel, A.J., Fleming, J.W., Player DJ., Martin Neil R. W., Lewis Mark P. Mechanical loading of tissue engineered skeletal muscle prevents dexamethasone induced myotube atrophy. J Muscle Res Cell Motil 42, 149–159 (2021). https://doi.org/10.1007/s10974-020-09589-0
Rimington, R.P., Capel, A.J., Chaplin K.F., Fleming, J.W., H.C.H Bandulasena, Bibb, R.J., Christie S.D.R., Lewis, M.P. Differentiation of Bioengineered Skeletal Muscle within a 3D Printed Perfusion Bioreactor Reduces Atrophic and Inflammatory Gene Expression. ACS Biomater. Sci. Eng. 2019, 5, 10, 5525–5538. https://doi.org/10.1021/acsbiomaterials.9b00975
Diana Maradze, Andrew Capel, Neil Martin, Mark P. Lewis, Yufeng Zheng, Yang Liu. In vitro investigation of cellular effects of magnesium and magnesium-calcium alloy corrosion products on skeletal muscle regeneration. Journal of Materials Science & Technology. Volume 35, Issue 11. 2019. Pages 2503-2512. https://doi.org/10.1016/j.jmst.2019.01.020
R. P. Rimington, A. J. Capel, D. J. Player, R. J. Bibb, S. D. R. Christie, M. P. Lewis. Feasibility and Biocompatibility of 3D-Printed Photopolymerized and Laser Sintered Polymers for Neuronal, Myogenic, and Hepatic Cell Types Macromol. Biosci. 2018, 18, 1800113. https://doi.org/10.1002/mabi.201800113
N. M. Wragg, D. Mosqueira, L. Blokpeol-Ferreras, A. Capel, D. J. Player, N. R. W. Martin, Y. Liu, M. P. Lewis, Development of a 3D Tissue-Engineered Skeletal Muscle and Bone Co-culture System. Biotechnol. J. 2019, 15, 1900106. https://doi.org/10.1002/biot.201900106
Turner, M. C. , Rimington, R. P. , Martin, N. R. W. , Fleming, J. W. , Capel, A. J. , Hodson, L. , & Lewis, M. P. (2021). Physiological and pathophysiological concentrations of fatty acids induce lipid droplet accumulation and impair functional performance of tissue engineered skeletal muscle. J Cell Physiol, 236, 7033–7044. https://doi.org/10.1002/jcp.30365
Marani L, Pardo-Figuerez M, Capel AJ, Nilsson Z, Stolzing A, Lewis MP. Development of a human in vitro model of the neuromuscular junction, using induced pluripotent stem cell-derived motor neurons and 3d tissue engineering constructs. Orthop Procs. 2018;100-B(SUPP_16):79-79. doi:10.1302/1358-992X.2018.16.079